Explaining the Differences in Affinity and of Carbohydrate Binding of the Glycogen-Sensing Enzyme, AMPK

The UBA domain of SnRK1 promotes activation and maintains catalytic activity

S Emanuelle, MS Doblin, PR Gooley, MS Gentry

2018-02-26

Sucrose non-fermenting 1-related protein kinase 1 (SnRK1) is a central metabolic regulator and the plant orthologue of the mammali..

Unravelling the Carbohydrate-Binding Preferences of the Carbohydrate-Binding Modules of AMP-Activated Protein Kinase

JI Mobbs, A Di Paolo, RD Metcalfe, E Selig, DI Stapleton, MDW Griffin, PR Gooley

2018-02-02

The β subunit of adenosine monophosphate (AMP)-activated protein kinase (AMPK), which exists as two isoforms (β1 and β2) in humans..

When phosphorylated at Thr148, the β2 -subunit of AMP-activated kinase does not associate with glycogen in skeletal muscle

H Xu, NT Frankenberg, GD Lamb, PR Gooley, DI Stapleton, RM Murphy

2016-07-01

The 5′- AMP-activated protein kinase (AMPK), a heterotrimeric complex that functions as an intracellular fuel sensor that affects ..

Molecular Insights into the Enigmatic Metabolic Regulator, SnRK1

S Emanuelle, MS Doblin, DI Stapleton, A Bacic, PR Gooley

2016-04-01

Sucrose non-fermenting-1 (SNF1)-related kinase 1 (SnRK1) lies at the heart of metabolic homeostasis in plants and is crucial for n..

Determinants of oligosaccharide specificity of the carbohydrate-binding modules of AMP-activated protein kinase

JI Mobbs, A Koay, A Di Paolo, M Bieri, EJ Petrie, MA Gorman, L Doughty, MW Parker, DI Stapleton, MDW Griffin, PR Gooley

2015-06-01

AMP-activated protein kinase (AMPK) is an αβγ heterotrimer that is important in regulating energy metabolism in all eukaryotes. Th..

Structure of amylase-binding protein A of Streptococcus gordonii: A potential receptor for human salivary α-amylase enzyme

A Sethi, B Mohanty, N Ramasubbu, PR Gooley

2015-06-01

Amylase-binding protein A (AbpA) of a number of oral streptococci is essential for the colonization of the dental pellicle. We hav..

SnRK1 from Arabidopsis thaliana is an atypical AMPK

S Emanuelle, MI Hossain, IE Moller, HL Pedersen, AML Van De Meene, MS Doblin, A Koay, JS Oakhill, JW Scott, WGT Willats, BE Kemp, A Bacic, PR Gooley, DI Stapleton

2015-04-01

Summary SNF1-related protein kinase 1 (SnRK1) is the plant orthologue of the evolutionarily-conserved SNF1/AMPK/SnRK1 protein kina..

Single fiber analyses of glycogen-related proteins reveal their differential association with glycogen in rat skeletal muscle

RM Murphy, H Xu, H Latchman, NT Larkins, PR Gooley, DI Stapleton

2012-12-01

To understand how glycogen affects skeletal muscle physiology, we examined enzymes essential for muscle glycogen synthesis and deg..

AMP-activated protein kinase β-subunit requires internal motion for optimal carbohydrate binding

M Bieri, JI Mobbs, A Koay, G Louey, YF Mok, DM Hatters, JT Park, KH Park, D Neumann, D Stapleton, PR Gooley

2012-01-18

AMP-activated protein kinase interacts with oligosaccharides and glycogen through the carbohydrate-binding module (CBM) containing..

Macromolecular NMR spectroscopy for the non-spectroscopist: Beyond macromolecular solution structure determination

M Bieri, AH Kwan, M Mobli, GF King, JP MacKay, PR Gooley

2011-03-01

A strength of NMR spectroscopy is its ability to monitor, on an atomic level, molecular changes and interactions. In this review, ..